Toxicokinetics of paraquat in rabbits.
- Author:
Xin KAN
1
;
Xiao-Yun ZHANG
;
Jie DONG
;
Wan-Shu LI
;
Guo-Xin HU
;
Zhong-Qiu LU
Author Information
- Publication Type:Journal Article
- MeSH: Administration, Oral; Animals; Biological Availability; Chromatography, High Pressure Liquid; Injections, Intravenous; Male; Paraquat; blood; pharmacokinetics; toxicity; Rabbits
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2010;28(10):756-759
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEto develop a high performance liquid chromatography method (HPLC) for the determination of paraquat in rabbit plasma and study its toxicokinetics in rabbits.
METHODStwelve rabbits were randomly divided into 2 groups with giving oral and intravenous administration of paraquat at a single dose of 60 mg/kg and 6 mg/kg respectively. The plasma paraquat concentrations were determined by HPLC and calculated by DAS pharmacokinetics program.
RESULTSthe linear range of paraquat in plasma was 0.05 ∼ 50.00 mg/L (r = 0.9998). The relative recoveries of the assay were 99.41% ∼ 102.32%. The absolute recoveries of the assay were 83.72% ∼ 90.48%. Both the intra-day and inter-day validations were less than 10%. For oral administration, the toxicokinetics parameters of paraquat were as follows: Cmax (14.46 ± 2.35) mg/L, Tmax (1.63 ± 0.31) h, AUC(0-t) (177.61 ± 14.62) mg × h/L, AUC(0-∞) (182.24 ± 14.54) mg × h/L, While for intravenous administration, the toxicokinetics parameters of paraquat: Cmax (35.13 ± 5.53) mg/L, Tmax 0.05 h, AUC(0-t) (121.74 ± 12.30) mg × h/L, AUC(0-∞) (125.12 ± 12.17) mg × h/L, The difference of these parameters between the two groups had statistical significance (P < 0.05). The oral bioavailability was (14.66 ± 1.55)%.
CONCLUSIONthe oral bioavailability of paraquat is relatively low. The biological half life of paraquat is relatively long and there is no significant difference between oral administration and intravenous on biological half life. This method is simple, sensitive and accurate. It can be used for the investigation of paraquat in rabbits.