Pirfenidone diminishes SiO2 induced lung fibrosis in rats.
- Author:
Ling-Fang FENG
1
;
Zhen-Yu JIA
;
Li-Jin ZHU
;
Li JU
;
Jun-Qiang CHEN
;
Zhao-Qiang JIANG
;
Ri-Ping CHEN
;
Zhen MA
;
Xing ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Hydroxyproline; metabolism; Lung; drug effects; metabolism; pathology; Male; Pulmonary Fibrosis; drug therapy; metabolism; pathology; Pyridones; pharmacology; therapeutic use; Rats; Rats, Sprague-Dawley; Silicon Dioxide; adverse effects
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2010;28(10):772-775
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEto investigate whether pirfenidone (PFD) presents the antifibrotic effect in silicosis of rats.
METHODSSD rats were randomly divided into five groups: the non-treat group, the normal saline group, the normal saline + PFD group, the SiO2 group, the SiO2 + PFD group. Rats except in the non-treat group were intratracheally instilled with SiO2 (25 mg/ml) or normal saline. The rats in normal saline + PFD group and the SiO2 + PFD group were given PFD (50 mg/kg) orally the next day after instillation and throughout the study. Rats were respectively sacrificed 7, 21, 42 days after instillation. The pathology changes were evaluated by Haematoxylin-eosin (HE), Van Gieson and Foot staining, and the hydroxyproline (HYP) content of pulmonary tissue was determined.
RESULTScompared with the SiO2 group, PFD could relieve the fibrotic changes in the lungs of rats. The fibrotic degree in silicotic lesions of lungs was lower in the SiO2 + PFD group than that of SiO2 group. The HYP content in the lungs of the SiO2 + PFD group [(0.75 ± 0.12) mg/g] was significantly lower than that of the SiO2 group [(1.19 ± 0.17) mg/g] at 42 days after instillation (P < 0.05).
CONCLUSIONthese data support that PFD has an antifibrotic effect against SiO2 induced lung fibrosis in rats, Which appears to be changing collagen accumulation and inhibiting pulmonary fibrosis.
