Change of structural maintenance of chromosome (SMC)1, SMC3, Separase and Securin expression in BEAS-2B malignant transformation cell induced by coal tar pitch smoke extracts.
- Author:
Zhi-Tao LI
1
;
Wei WANG
;
Yong ZHAO
;
Li-Xia WANG
;
Han-Song ZHU
;
Wei-Dong WU
;
Yi-Ming WU
Author Information
- Publication Type:Journal Article
- MeSH: Bronchi; cytology; Cell Cycle Proteins; metabolism; Cell Line; Cell Line, Transformed; cytology; drug effects; Chondroitin Sulfate Proteoglycans; metabolism; Chromosomal Proteins, Non-Histone; metabolism; Coal Tar; toxicity; Endopeptidases; metabolism; Epithelial Cells; cytology; drug effects; metabolism; Humans; Membrane Proteins; metabolism; Separase; Sister Chromatid Exchange; Smoke; adverse effects
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2010;28(10):776-780
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEto study the role of structural maintenance of chromosome (SMC)1, SMC3, Separase and Securin in tumorgenesis that contact with coal tar pitch.
METHODSthe BEAS-2B cells was induced by coal tar pitch smoke extracts to form malignant transformation cell model in vitro. The gene expression levels of mRNA were assessed by real-time quantitative RT-PCR, and the protein expression variation were determined by cell culture overslip of immunohistochemical methods.
RESULTSin malignant transformation cells, the mRNA and the protein expression level of SMC1 gene was not statistically significantly different compared with the BEAS-2B group and DMSO group (P > 0.05); SMC3 and Separase was increased and Securin was decreased (P < 0.05), while the difference between other two control groups was not significant (P > 0.05).
CONCLUSIONSthe up expression level of SMC3 and Separase and the down expression level of Securin are involved in the process that evolves into malignant transformation in bronchial epithelial cells BEAS-2B induced by coal tar pitch smoke extracts.
