Effect of reactive oxygen species during the leukemogenic process associated with exposure to benzene.
- Author:
Yi-min LIU
1
;
Yong-sheng LI
;
Xu-dong LI
;
Xiao GUO
Author Information
- Publication Type:Journal Article
- MeSH: Benzoquinones; toxicity; Cell Proliferation; drug effects; HL-60 Cells; drug effects; metabolism; pathology; Humans; Reactive Oxygen Species; metabolism
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2009;27(12):738-740
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of reactive oxygen species (ROS) during the leukemogenic process associated with exposure to benzene.
METHODSHL-60 was treated with 3 micromol/L benzoquinone (BQ). Generation of ROS in cells was measured by DCFH-DA method. For proliferation assays,cells were stained with alamar blue dye and counted.
RESULTSROS production and the proliferation of cell were all increased in BQ-treated cells (13.10 +/- 0.15, 185% +/- 30.00%) as compared with control cells (11.32 +/- 0.09, 100% +/- 0.00%) (P < 0.05); The addition of catalase just before BQ addition reduced ROS generation to basal levels and decreased the growth of cell (P < 0.05).
CONCLUSIONROS may play an important role in the process of proliferation of HL-60 cells induced by BQ.
