Construction of Human ScFv Phage Display Library against Ovarian Tumor
- Author:
Jinsong XIA
1
;
Hao BI
;
Qin YAO
;
Shen QU
;
Yiqiang ZONG
Author Information
1. 华中科技大学同济医学院附属同济医院
- Keywords:
ScFv phage display library;
ovarian tumor;
SOE;
radioimmunoimaging
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2006;26(5):497-499
- CountryChina
- Language:Chinese
-
Abstract:
In order to construct a single chain fragment variable (ScFv) phage display library against ovarian tumor, by using RT-PCR, the human heavy chain variable region genes (VH) and light chain variable region genes (VL) were amplified from lymphocytes of ovarian tumor patients and subsequently assembled into ScFv genes by SOE. The resulting ScFv genes were electrotransformed into E.coli TG1 and amplified with the co-infection of helper phage M13KO7 to obtain phage display library. The capacity and titer of the resulting library were detected. The phage antibody library with a capacity of approximately 3 × 109 cfu/μg was obtained. After amplification with helper phage, the titer of antibody library reached 5 × 1012 cfu/mL. Human ScFv library against ovarian tumor was constructed successfully, which laid a foundation for the screening of ovarian tumor specific ScFv for the radioimmunoimaging diagnosis of ovarian tumor.
