A Novel Frequent BRCA1 Allele in Chinese Patients with Breast Cancer
- Author:
Dongxian ZHOU
1
;
Wen XIONG
;
Hongxan XU
;
Chaopeng SHAO
Author Information
1. 广东省深圳市第一人民医院
- Keywords:
BRCA1;
sequencing;
single nucleotide polymorphism;
PCR-SSP;
Chinese
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2006;26(5):576-579
- CountryChina
- Language:Chinese
-
Abstract:
The whole length of exon 11 of BRCA1 was sequenced (total 3427 bp) in 59 patients and 10 healthy female blood donors. To allow a rapid determination of the different BRCA1 alleles, a sequence-specific primer PCR method (PCR-SSP) was established and was applied to 57 additional female donors. Finally, the full-length coding region of BRCA1 was analyzed through reversed-transcriptase PCR (RT-PCR) and cDNA sequencing (total 5554 bp) in one donor with wild-type allele and 2 patients with one or two mutated alleles. By genomic DNA sequencing, 5 homozygous polymorphisms were observed in 18 patients: 2201C＞T, 2430T＞C, 2731C＞T, 3232A＞G and 3667A＞G. All of them were previously observed in Caucasians, Malay and Chinese, but for the first time the mutations were found in one allele (GenBank AY304547). Twenty-six patients and 4donors were heterozygous at these 5 nucleotide positions. The remaining 15 patients and 6 donors showed a sequence identical with the standard BRCA1 gene. Combined the PCR-SSP results and in a summary, 6 of 67 (9.0 %) healthy individuals were homozygous for the mutated allele, whereas 18 of 59 (30.5 %) breast cancer patients were homozygous. A Chi-square test showed a significant correlation between homozygous mutated BRCA1 allele and breast cancer. The cDNA sequencing showed that 2 additional mutations, 4427T＞C in exon 13 and 4956A＞G in exon 16, were found. A new BRCA1 allele, which is BRCA1-2201T/2430C/2731T/3232G/3667G/4427C/4956G (GenBank AY751490), was found in Chinese. And the homozygote of this mutated allele may implicate a disease-association in Chinese.
