Vascular Endothelial Growth Factor165-regulated Nasopharyngeal Carcinoma Cell Lines Invasion and Migration Involve Expression and Activation of Matrix Metalloproteinase-2
- Author:
Yanjun WANG
1
;
Weijia KONG
;
Jianxin YUE
;
Dawei SUN
;
Wei LI
;
Qi YAO
;
Yu SUN
;
Jihua DONG
Author Information
1. 华中科技大学同济医学院附属协和医院
- Keywords:
nasopharyngeal carcinoma cells;
vascular endothelial growth factor;
matrix metalloproteinase-2;
gene transfection
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2006;26(5):621-624
- CountryChina
- Language:Chinese
-
Abstract:
The effect of vascular endothelial growth factor (VEGF) overexpression on matrix metalloproteinase-2 (MMP-2) in nasopharyngeal carcinoma (NPC) cells in vitro and the possible mechanism involved were investigated, and the correlation between the expression of VEGF and MMP-2 in NPC evaluated. The NPC cells were transfected with PAd-trackVEGF165 plasmid. The expression levels of VEGF and MMP-2 mRNA and protein in NPC cells were detected by semi-quantitative RT-PCR and Western blot respectively. It was found that the expression of VEGF and MMP-2 mRNA and protein was significantly increased in NPC cells after transfection of VEGF165. It was concluded that the expression of VEGF was correlated to the in vitro invasion of NPC cells, and the induction of MMP-2 by VEGF was a key process of NPC cell invasion.
