Intrastriatal Gene Transfer of Vascular Endothelial Growth Factor Rescues Dopaminergic Neurons in a Rat Parkinson's Disease Model
- Author:
Youyong TIAN
1
;
Shenggang SUN
;
Cuiju TANG
;
Jianing WANG
;
Xiaowu CHEN
;
Xian QIAO
Author Information
1. 同济医学院附属协和医院
- Keywords:
vascular endothelial growth factor A;
Parkinson's disease;
gene therapy;
gene transfer technique
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2006;26(6):670-673
- CountryChina
- Language:Chinese
-
Abstract:
To examine the ability of intrastriatal gene transfer of vascular endothelial growth factor 165 mediated by adenoviral vector to rescue dopaminergic neurons in a rat model of Parkinson's disease (PD), we constructed recombinant replication-deficent adenoviral vectors carrying the gene of VEGF165 (Ad-VEGF), and injected Ad-VEGF (or Ad-LacZ and PBS as controls) into the striatum of rats 7 days after the lesion by 6-hydroxydopamine. The rat rotational behavior analysis and tyrosine hydroxylase (TH) immunohistochemistry were performed to assess the change of dopaminergic neurons. Our results showed that the rats receiving Ad-VEGF injection displayed a significant improvement in apomorphine-induced rotational behavior and a significant preservation of TH-positive neurons and fibers compared with control animals. It is concluded that intrastriatal gene transfer by Ad-VEGF may rescue the dopaminergic neurons from degeneration in a rat model of PD.
