Liver Pathology after Transplantation of Short-Term Preserved Canine Liver with Euro-Collins or UW Solution.
- Author:
Ku Yong CHUNG
1
;
Myoung Soo KIM
;
Yoon Seok CHAE
;
Jong Hoon LEE
;
Soon Hee SUNG
;
Kum Ja CHOI
;
Woo Jung LEE
Author Information
1. Research Institute for Transplantation, Yonsei University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Liver pathology;
Experimental liver transplantation;
Preservation solution;
Euro-Collins solution;
UW solution
- MeSH:
Alkaline Phosphatase;
Animals;
Aspartate Aminotransferases;
Biopsy;
Dogs;
Hepatectomy;
Humans;
L-Lactate Dehydrogenase;
Liver Transplantation;
Liver*;
Living Donors;
Pathology*;
Reperfusion;
Survival Rate;
Tissue Donors;
Transplants;
Wisconsin
- From:The Journal of the Korean Society for Transplantation
2001;15(2):134-138
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: In living donor partial liver transplantation, the organ cold preservation time is relatively short, thereby, Euro-Collins (EC) solution could be used with comparable result to University of Wisconsin (UW) solution. METHODS: About 70% partial liver was harvested and reimplanted after 3.5 hours of cold preservation with EC or UW solution in same mongrel dog weighing about 25 kg. In 5 cases EC solutions were used, and UW solutions were used in another 5 cases. Blood sample and liver biopsy specimens were taken just before donor hepatectomy, postreperfusion 1 hour, 7 days and 14 days after operation. Serum aspartate aminotransferase (AST), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) were monitored, and morphologic grading of liver injury was performed as Spiegel et al. reported in 1999. RESULTS: Total ischemic time was 4 hours and 27 minutes in average. At 1 hour after reperfusion, there were no significant differences in liver enzyme and morphological score between the UW solution group and the EC group. Survival rate was similar in two groups; 2 dongs were alive at postoperative 7th day, and 1 dog was alive at postoperative 14th day in each group. CONCLUSION: In living donor partial liver transplantation where both operations could be performed simultaneously with relatively short graft preservation time, EC solution could be used with comparable results to UW solution.
