Pancreatic Islet Cell Autotransplantation in the Canine Model.
- Author:
Jae Berm PARK
1
;
Seong Eun CHON
;
Jae Hoon PARK
;
Kwang Woong LEE
;
Jeong Han KIM
;
In Kyung JEONG
;
Yong Il KIM
;
Jae Won JOH
;
Suk Koo LEE
;
Byung Boong LEE
;
Kwang Won KIM
;
Sung Joo KIM
Author Information
1. Department of Surgery, Samsung Medical Center Sungkyunkwan University School of Medicine, Seoul, Korea. sjkim@smc.smasung.co.kr
- Publication Type:Original Article
- Keywords:
Islet cell autotransplantation;
Pancreas
- MeSH:
Adult;
Animals;
Autografts*;
Dogs;
Graft Survival;
Humans;
Insulin;
Islets of Langerhans Transplantation;
Islets of Langerhans*;
Pancreas;
Pancreas Transplantation;
Pancreatectomy;
Transplantation, Autologous;
Transplants
- From:The Journal of the Korean Society for Transplantation
2001;15(2):139-141
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
INTRODUCTION: Islet cell transplantation is an attractive alternative to whole organ pancreas transplantation, because it is clearly the safer and simpler surgical procedure for recipients. PURPOSE: The authors intended to examine the functional outcome of islet autografting and the factors related to islet graft survival in mongrel dogs. METHODS: Eighteen adult mongrel dogs were used for the study of total pancreatectomy and islet autotransplantation. The harvested total pancreas was preserved in iced Hank's balanced salt solution (HBSS). The islets were properly isolated by a modified Recordi method. RESULTS: The median volume of the transplanted pancreatic islet tissue was 2.1 mL (range 0.7 to 5.0) and purity was 63% (range 10 to 95). Twelve dogs from the experimental group having undergone successful autografting of islets were followed for a minimum of 6 weeks. Three of the 12 dogs maintained insulin secretory function for 6 weeks and the other 7 dogs showed normal Kg values for 6 months following islet transplantation. In the remaining 2 cases, islet graft function was sustained for 1 year. The median required insulin dosage for maintenance of normal FPG was 0.7 U/kg per day (range 0 to 1.6). The insulin requirement correlated well with the IEq/ kg level (r=.90, P<.01). Dogs with >6,000 IEq/kg had a better chance of being insulin-independent. CONCLUSION: In this study, we confirmed that autotransplantation of islet cells on pancreatectomized dogs can render nearly normoglycemia, and islet transplantation dogs can be used as a model for the assessment of insulin secretory function.
