The Effects of Glutathione on Cyclosporine Induced Cytotoxicity in Cultured Insulinoma Cells.
- Author:
Byung Cheol KIM
1
;
Seong Hwan KIM
;
Young Don MIN
;
Jeong Hwan CHANG
Author Information
1. Department of Surgery, College of Medicine, Chosun University, Gwangju, Korea.
- Publication Type:Original Article
- Keywords:
Rat insulinoma (RINm5F) cells;
Cyclosporine A;
Glutathione
- MeSH:
Animals;
Buthionine Sulfoximine;
Cell Survival;
Cyclosporine*;
Cysteine;
Glutathione*;
Humans;
Hypertension;
Insulinoma*;
Ligases;
Rats;
Reactive Oxygen Species
- From:The Journal of the Korean Society for Transplantation
2001;15(2):147-150
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Cycloporine A (CsA) is a immunosuppressive agent most widely used in organ transplanted patients for preventing immunorejection, but it has some side effects, including hypertension, nephrotoxicity, hepatotoxicity and diabetes. The mechanism of toxicity of CsA was not completely understood, but the reactive oxygen species has been proposed to be involved in the reaction of toxicity of CsA. The purpose of this study is to determine the effects of glutathione, as a physiological antioxidant on CsA induced cytotoxicity in rat insulinoma (RINm5F) cells. METHODS: RINm5F cells were incubated in the presence of CsA (105~108 M) and buthionine sulfoximine (BSO), as a inhibitor of r-glutamyl cysteine synthetase, was added to cultured media (RPMI1640). Twenty four hours of incubation with CsA and BSO, viable cells were determined by MTT method. RESULTS: CsA decreased cell viability in dose response in cultured RINm5F cells and significantly decreased according to redusing glutathione. CONCLUSION: These results suggested that CsA may induce the diabetes and glutathione have some roles in the pathogenesis of CsA-induced diabetes.
