Apoptosis inducing effect of Hechanpian on human lung adenocarcinoma A549 cells.
- Author:
Shao-Quan XIONG
1
;
Dai-Han ZHOU
;
Li-Zhu LIN
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; pathology; Animals; Antineoplastic Agents, Phytogenic; pharmacology; Apoptosis; drug effects; Cell Line, Tumor; Down-Regulation; Drugs, Chinese Herbal; pharmacology; Female; Humans; Lung Neoplasms; pathology; Male; RNA, Messenger; genetics; metabolism; Rats; Rats, Sprague-Dawley; Receptor, Epidermal Growth Factor; genetics; metabolism
- From: Chinese Journal of Integrated Traditional and Western Medicine 2010;30(6):607-610
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the apoptosis inducing effects of Hechanpian (HCP) on human lung adenocarcinoma A549 cells.
METHODSHCP containing rat serum was prepared and applied on A549 cells. The cell growth inhibition rate was tested by MTT assay; the effect of HCP on cell apoptosis was observed with Propidium iodide (PI) staining and flow cytometry analysis; the mRNA expression of epidermal growth factor receptor (EGFR) was detected through RT-PCR.
RESULTSThe growth of A549 cells was obviously inhibited after being treated by HCP containing serum, and the cells presented an apoptotic change. The cell apoptosis rate after treated by serum containing 10% and 20% HCP was 20.5% and 33.2%, respectively, significantly higher than that in the control (6.1% in cells didn't treated with HCP, P < 0.05). Compared with control, EGFR mRNA expression in HCP treated cells was significantly lower (P < 0.05).
CONCLUSIONHCP has apoptosis inducing effect on A549 cell, and its molecular mechanism is probably correlated with the inhibition of EGFR gene transcription.
