Effect of depside salt from salvia miltiorrhizae in repairing advanced glycation end products-induced late endothelial progenitor cell dysfunction and its molecular mechanism.
- Author:
Qin CHEN
1
;
Ming-Han HUANG
;
Shi-Sen JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Apoptosis; drug effects; Cell Movement; drug effects; Cells, Cultured; Depsides; isolation & purification; pharmacology; Endothelium, Vascular; cytology; drug effects; metabolism; Female; Glycation End Products, Advanced; antagonists & inhibitors; pharmacology; Humans; Nitric Oxide Synthase Type III; metabolism; Proto-Oncogene Proteins c-akt; metabolism; Receptor for Advanced Glycation End Products; Receptors, Immunologic; metabolism; Salvia miltiorrhiza; chemistry; Stem Cells; drug effects; metabolism; physiology; Young Adult
- From: Chinese Journal of Integrated Traditional and Western Medicine 2010;30(6):630-635
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of depside salt from salvia miltiorrhizae (DSSM) in repairing advanced glycation end products (AGE)-induced late endothelial progenitor cell (EPC) dysfunction, and its possible molecular mechanism.
METHODSMononuclear cells (MNCs) were separated using density gradient centrifugation from human umbilical cord blood, and cultured with EGM-2-MV culture fluid to late EPCs. Then the EPCs were divided into 5 groups: Group A incubated with 200 microg/mL AGE-modified bovine serum albumin (AGE-albumin) alone (A), Groups B, C and D with equal dosage of AGE-albumin plus DSSM at different dosages (0.1 microg/mL, 1 microg/mL, and 10 microg/mL), Group E with 200 microg/mL of unmodified-AGE. The late EPCs apoptosis was detected by Annexin V+/PI double-stain, angiogenic capacity was detected by ECMatrix-gel, mRNA expressions of the receptor for AGE (RAGE) and endothelial nitric oxide synthase (eNOS) were measured by reverse-transcriptase polymerase chain reaction (RT-PCR), and the protein expressions of RAGE, eNOS and protein kinase (Akt) were measured by Western blot.
RESULTSCompared with Group E, in Group A, the Annexin V+/PI- ratio and expression of RAGE in EPCs increased, the angiogenic capacity, mRNA and protein expressions of eNOS, and protein expression of Akt decreased significantly. These abnormal changes in Groups C and D were significantly smaller than those in Group A (P < 0.05 or P < 0.01). And all the indices in Group D were adjacent to those in Group E, showing insignificant difference between the two groups (P > 0.05).
CONCLUSIONSAGE could injure the function of EPCs, revealing increase of cell apoptosis and migration, deprivation of angiogenic capacity in vitro. DSSM could repair the EPCs dysfunction induced by AGE-albumin. Up-regulation of eNOS and Akt in these cells may be involved in the mechanism.