Effect of Shenqi compound on PTEN/PI3K signal transduction in GK rats with diabetes mellitus macroangiopathy.
- Author:
Ya LIU
1
;
Chun-Guang XIE
;
Min CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Angiogenesis Inhibitors; pharmacology; therapeutic use; Animals; Aorta; metabolism; Astragalus membranaceus; chemistry; Atherosclerosis; prevention & control; Class Ia Phosphatidylinositol 3-Kinase; genetics; metabolism; Diabetes Mellitus, Type 2; complications; drug therapy; metabolism; Diabetic Angiopathies; drug therapy; Drugs, Chinese Herbal; administration & dosage; pharmacology; therapeutic use; Male; PTEN Phosphohydrolase; genetics; metabolism; Panax; chemistry; Phytotherapy; RNA, Messenger; genetics; metabolism; Rats; Rats, Inbred Strains; Rats, Wistar; Signal Transduction; drug effects
- From: Chinese Journal of Integrated Traditional and Western Medicine 2010;30(6):640-644
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo research the effects and mechanism of Shenqi compound (SQC) on PTEN/ PI3K signal transducing path and angiogenesis in Goto-Kakizaki (GK) rats with diabetes mellitus type 2 (DM2) caused macroangiopathy.
METHODGK rats with blood sugar > or = 11.1 mmol/L were divided into 4 groups, the GK group, the model group, the Western medicine (WM) group treated by atorvastatin 1.5 mg/(kg x d) and the Chinese medicine (CM) group treated with SQC 1.44 g/(kg x d). All were fed 35 days with high fatty diet, but to the latter three groups, N omega-nitriyl-L-arginine methyl ester 0.1 mg/mL was added into their drinking water for macroangiopathy model establishing. Besides, a group of normal Wistar rats fed with ordinary forage was set for control. Rat's blood glucose and lipids were measured, morphology of abdominal aorta wall tissue was observed with HE staining, and mRNA expressions of PTEN and PI3Kp85 in aortic wall were detected by Real-time PCR.
RESULTSGeneral condition, gluco-lipid metabolism and aortic morphology in the CM group were significantly better than those in the model group. PTEN mRNA expression in the CM group (1.10 +/- 0.48) was significantly higher than that in the GK group (0.63 +/- 0.16) and the model group (0.17 +/- 0.07, both P < 0.01), but near to that in the WM group (1.11 +/- 0.46), while the PI3Kp85 mRNA expression in the TCM group (0.19 +/- 0.05) was lower than that in the GK group (1.38 +/- 0.43, P < 0.01), but near to that in the model group (0.33 +/- 0.09) and the WM group (0.11 +/- 0.06, both P > 0.05).
CONCLUSIONSQC could increase the PTEN mRNA expression and restrain the PI3Kp85 mRNA expression in aorta, which is possibly the partial mechanisms of action of the remedy in inhibiting angiogenesis and preventing diabetic macroangiopathy.
