Inhibitory effect of matrine on proliferation of human ovary malignant teratoma cell line PA-1 in vitro.
- Author:
Cong-de CHEN
1
;
Xiang LI
;
Hao-chuan ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Alkaloids; pharmacology; Antineoplastic Agents, Phytogenic; pharmacology; Cell Cycle; drug effects; Cell Line, Tumor; Cell Proliferation; drug effects; Female; Humans; Ovarian Neoplasms; pathology; Proto-Oncogene Proteins c-bcl-2; genetics; metabolism; Quinolizines; pharmacology; RNA, Messenger; genetics; metabolism; Teratoma; pathology; bcl-2-Associated X Protein; genetics; metabolism
- From: Chinese Journal of Integrated Traditional and Western Medicine 2010;30(7):723-725
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of matrine (MAT) on the proliferation of human ovary malignant teratoma cell line PA-1 in vitro.
METHODSPA-1 cells allocated in different groups were treated with different concentrations (0.25 mg/mL, 0.5 mg/mL and 1.0 mg/mL) of MAT. The inhibitory effect of MAT and its dose- and time-effect relationship were detected with MTT; the apoptosis rate and cell cycle were evaluated by flow cytometry; and the changes of bcl-2/bax mRNA expression in cells were measured using semi-quantitative RT-PCR.
RESULTSAfter being exposed to MAT, the PA-1 cell proliferation was decreased in a concentration- and time-dependent manner; cell apoptosis rate raised as the increasing concentration of MAT and acting time; cells retarded at G1 phase in the cell cycle dose-dependently; and the bcl-2/bax mRNA expression in cells dawn-regulated significantly.
CONCLUSIONMAT can dose- and time-dependently inhibit the proliferation of PA-1 cell by reducing bcl-2/bax mRNA ratio to produce a G1 phase arresting in cell cycle.
