Effect of dureping injection on T-cells function and their function in killing FM1 infected Mphi in vitro.
- Author:
Shan JING
1
;
Li-gang GU
;
Yun ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Adjuvants, Immunologic; pharmacology; Animals; Antiviral Agents; pharmacology; Cells, Cultured; Drugs, Chinese Herbal; pharmacology; Influenza A virus; drug effects; Interferon-gamma; immunology; metabolism; Interleukin-10; immunology; metabolism; Mice; Mice, Inbred BALB C; Orthomyxoviridae Infections; immunology; Spleen; cytology; T-Lymphocytes; cytology; immunology; virology
- From: Chinese Journal of Integrated Traditional and Western Medicine 2010;30(7):729-732
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of Dureping Injection (DRP) on the T-cells function of mice and the function of T-cells in killing MF infected by influenza virus subtype A mice-lung adaptive strain FM1 in vitro.
METHODSNumber of splenic normal and FM1 infected T-cells in mice were measured by MTT and double-antibody sandwich ELISA, after being treated with DRP at different concentrations (2.1, 8.5 and 17.0 microg/mL), and the effect of DRP on interferon-gamma (IFN-gamma) and interleukin-10 (IL-10) production as well as on splenic T-cell killing FM1 infected Mphi/Ana-1 function were detected.
RESULTSDRP inhibited the multiplication of normal spleen T cells induced by concanavalin A in vitro, suppressed Th2 cell factor IL-10 production, and maintained Th1 cell factor IFN-y at a definite level, moreover, it directly enhanced the power of T-cells in killing FM1 infected Mphi (P < 0.05, P < 0.01).
CONCLUSIONDRP could act on mice T-cells to enhance the immune response for antiinfluenza viral FM1 in vitro.
