Effect of Nervilla fordii on lung aquaporin 1 and 5 expression in endotoxin-induced acute lung injury rat.
- Author:
Yin-ji XU
1
;
Yuan-bin CHEN
;
Li-li WANG
Author Information
- Publication Type:Journal Article
- MeSH: Acute Lung Injury; chemically induced; drug therapy; prevention & control; Animals; Aquaporin 1; metabolism; Aquaporin 5; metabolism; Drugs, Chinese Herbal; therapeutic use; Endotoxins; Female; Lung; metabolism; Male; Phytotherapy; Rats; Rats, Sprague-Dawley; Up-Regulation
- From: Chinese Journal of Integrated Traditional and Western Medicine 2010;30(8):861-866
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the protective mechanism of Nervilia fordii (NF) by observing the effect of its pretreatment on lung aquaporin 1 and 5 (AQP-1, AQP-5) expression in rats with endotoxin-induced acute lung injury (ALI).
METHODSTwenty-four SD rats were randomly divided into 3 groups, the normal group (A), the NF pre-intervention group (B) and the endotoxin model group (C). Rats in Group B and C were made into ALI by endotoxin (5 mg/kg) injection via sublingual vein, and NF pretreatment was applied to Group B. Animals were sacrificed at the 8 h after modeling, their lung were taken for observing the water permeability change by wet/dry weight ratio (W/D) measuring, pathological feature by HE staining, and the expression of AQP-1, AQP-5 was detected by immunohistochemistry and RT-PCR.
RESULTSThe W/D ratio of lung was higher in model rats than in normal rats, but as compared with Group C, it was significantly lower (P < 0.05) in Group B. The pulmonary edematous change was significantly mild and the AQP-1 and AQP-5 protein expressions were significantly higher in Group B than in Group C (P < 0.05).
CONCLUSIONNF pretreatment can promote lung AQP-1 and AQP-5 expression up-regulation, increase lung water clearance and transportation to improve the water balance and eliminate pulmonary edema, so as to effectively protect lung from acute injury.
