Effect of precondition with GBE50 and Salviae miltionrrhizae on cycloxygenase-2 and its downstream effectors contents in ischemia/reperfusion myocardium.
- Author:
Yi-Min BAO
1
;
Ai-Hua LIU
;
Zhi-Xiong ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: 6-Ketoprostaglandin F1 alpha; metabolism; Animals; Cyclooxygenase 2; genetics; metabolism; Drugs, Chinese Herbal; therapeutic use; Ginkgo biloba; chemistry; Ischemic Preconditioning, Myocardial; methods; Male; Myocardial Ischemia; physiopathology; Myocardial Reperfusion Injury; metabolism; pathology; Myocardium; metabolism; RNA, Messenger; genetics; metabolism; Rats; Rats, Sprague-Dawley; Salvia miltiorrhiza; chemistry; Thromboxane B2; metabolism
- From: Chinese Journal of Integrated Traditional and Western Medicine 2010;30(10):1056-1060
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the changes in contents of cycloxygenase-2 (COX-2) and its downstream effectors in rat's myocardial ischemia/reperfusion (I/R) model and observe the effects of precondition with GBE50 (Ginkgo biloba extract 50) and Salviae miltiorrhizae (SM) on them.
METHODSRat's I/R model was established by 30-min left anterior descending coronary artery occlusion followed with 60-min reperfusion. Animals were divided into the model control group, the sham-operated group and the tested groups (received 1-week precondition with GBE50 and SM respectively via intragastric infusion before modeling). COX-2 mRNA expression in myocardium was detected by real-time PCR; contents of thromboxane B2 (TXB2) and 6-keto-prostaglandin F1alpha (6-keto-PGF1alpha) were measured by radioimmunoassay.
RESULTSThe mRNA expression of COX-2 in the model group was obviously higher than that in the sham-operated group (P < 0.001), while that in the tested groups was down-regulated significantly (P < 0.01), and the content of TXB2 as well as the ratio of TXB2/PGF1alpha was reduced significantly (P < 0.05). Besides, SM also showed the up-regulation effect on 6-keto-PGF1alpha content in myocardium (P < 0.05).
CONCLUSIONCOX-2 affects the myocardium through thromboxane A2 and prostacyclin after I/R; both GBE50 and SM can inhibit the production of COX-2, but they may act in different paths.
