Development of a protocol on pulsed field gel electrophoresis analysis for Streptococcus suis
10.3321/j.issn:0254-6450.2008.05.013
- VernacularTitle:猪链球菌脉冲场凝胶电泳分析方法的建立
- Author:
Li-Li WANG
1
;
Chang-Yun YE
;
Yan-Mei XU
;
Zhi-Gang CUI
;
Huai-Qi JING
;
Dong JIN
;
Hua-Mao DU
;
Shou-Yin ZHANG
;
Xue-Mei BAI
;
Ai-Lan ZHAO
;
Jian-Guo XU
Author Information
1. 北京市疾病预防控制中心
- Keywords:
Streptococcus suis;
Pulsed field gel electrophoresis analysis
- From:
Chinese Journal of Epidemiology
2008;29(5):473-477
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a PFGE protocol for Streptococcus suis.Methods We developed and optimized a PFGE protocol for S.suis,in terms of plug preparation,choice of restriction endonucleases and optimized electrophoresis parameters.By analyzing the genome sequences of S.suis P1/7 with Mapdraw of DNAStar.we found three restriction enzymes,Swa Ⅰ,Sma Ⅰ and Apa Ⅰ,were more suitable than others.Results Analysis of 100 isolates of S.suis including 34 of 35 serotypes identified,59,53 and 43 patterns were obtained from Swa Ⅰ,Sma Ⅰ and Apa Ⅰ restriction,respectively.The enzyme Swa Ⅰ had the greatest power for discrimination ability.Conclusion By optimization of the protocol at various conditions,a rapid,reproducible,economic and practical PFGE method for S.suis was developed.
