Studies on simultaneous detection of four foodborneviruses by multiplex reverse transcription-polymerase chain reaction

VernacularTitle:四种食源性病毒多重反转录-聚合酶链反应检测研究
Author: Xiao-Xia KOU ¹ ; Qing-Ping WU ; Lin YAO ; Ju-Mei ZHANG
Author Information

1. 广东省微生物研究所
Keywords: Foodbornevirus; Multiplex reverse transcription-polymerase chain reaction; Detection
From: Chinese Journal of Epidemiology 2008;29(6):590-593
CountryChina
Language:Chinese
Abstract: Objective To establish a method for simultaneous detection of norovirus (NV),rotavirus (RV), astrovirus (AV) and hepatitis A virus (HAV) by multiplex reverse transcriptionpolymerase chain reaction (RT-PCR). Methods Specific primers of the four viruses were designed based on the high conserved sequences, the reaction system and conditions optimized and the specificity and sensitivity confirmed. The method was then applied to detect the four viruses in clinical samples. Results The steady detection limits were 100 pg/ml for hepatitis A virus, 50 pg/ml for rotavirus, norovirus and astrovirus respectively. When the developed method was used to detect clinical fecal samples, 62(48.44%)were iden tified as rotavirus, 8 (6.25%) as norovirus, 11 (8.59%) as astrovirus and 4 (3.12%) as hepatitis A virus in a total of 128 samples. Conclusion Data from our study showed that multiplex RTPCR system could be used to simultaneously detect the four viruses in routine monitoring and risk assessment in disease outbreaks with high specificity and sensitivity.