E. coli-based production of recombinant FALL-39.
- Author:
Yun FENG
1
;
Yunxia YANG
;
Ning HUANG
;
Qi WU
;
Boyao WANG
Author Information
1. Research Unit of Infection & Immunity, West China Medical Center of Sichuan University, Chengdu 610041.
- Publication Type:Journal Article
- MeSH:
Escherichia coli;
genetics;
Genetic Vectors;
genetics;
Humans;
Peptide Biosynthesis;
Peptides;
genetics;
isolation & purification;
pharmacology;
Recombinant Proteins;
biosynthesis;
genetics;
isolation & purification;
pharmacology
- From:
Journal of Biomedical Engineering
2003;20(4):634-637
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed at constructing a prokaryotic expression system to resolve the difficulties in acquiring antibacterial peptide and to meet the needs of research and drug development. Total RNA was extracted from human pulmonary gland epithelial cell line SPC-A-1, and a cDNA encoding mature FALL-39 peptide was amplified by RT-PCR. The recombinant prokaryotic expression vector pGEX-1 lambda T-FALL-39 was constructed. Using affinity chromatography, thrombin cleaving and AU-PAGE elution, we obtained the purified FALL-39. MIC, MEC, MBC analyses demonstrated that the FALL-39 had strong antibacterial activity.
