Effects of recombinant bovine basic fibroblast growth factor on osteoblast's growth and c-fos expression.
- Author:
Yong GUO
1
;
Xizheng ZHANG
;
Yunshan ZHAO
;
Ruixin LI
;
Yuxia SHI
;
Jiming WU
Author Information
1. Institute of Medical Equipment, Academy of Military Sciences, Tianjin 300161, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cattle;
Cell Proliferation;
drug effects;
Cells, Cultured;
Fibroblast Growth Factor 2;
pharmacology;
Gene Expression Regulation;
drug effects;
Genes, fos;
drug effects;
Osteoblasts;
cytology;
drug effects;
metabolism;
Proto-Oncogene Proteins c-fos;
metabolism;
Rats;
Rats, Wistar;
Recombinant Proteins;
pharmacology
- From:
Journal of Biomedical Engineering
2004;21(1):8-11
- CountryChina
- Language:Chinese
-
Abstract:
Rat's osteoblasts cultured in vitro were stimulated by recombinant bovine basic fibroblast growth factor(rb-bFGF). After 1-2 days, the osteoblast grew long protuberance, the numbers of osteoblasts were greater than the control groups', the vitality of osteoblasts was better than that of control groups. After 1 hour, the expression of c-fos in osteoblast increased when compared with that in the control group. After the osteoblasts having been stimulated for 1-2 days, the expression of c-fos increased more conspicuously. These results show that bFGF can boost the osteoblast to grow and proliferate and can enhance the expression of c-fos gene. The increase of c-fos gene's expression may be an important step in the signal transformation process of all kinds of stimulation boosting osteoblast to proliferate.
