Effect of Shengji Huayu Recipe on the expression of MMP-3 and TIMP-1 in skin ulcer tissue of diabetic rats.
- Author:
Yi-Fei WANG
;
Xin LI
;
Rong XU
;
Wen-Cheng JIANG
;
Fu-Lun LI
;
Kan ZE
;
Bin LI
- Publication Type:Journal Article
- MeSH: Animals; Diabetes Mellitus, Experimental; metabolism; Drugs, Chinese Herbal; pharmacology; therapeutic use; Male; Matrix Metalloproteinase 3; metabolism; Phytotherapy; Rats; Rats, Sprague-Dawley; Skin; drug effects; pathology; Skin Ulcer; drug therapy; metabolism; Tissue Inhibitor of Metalloproteinase-1; metabolism
- From: Chinese Journal of Integrated Traditional and Western Medicine 2014;34(2):218-223
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of Shengji Huayu Recipe (SHR)on the expression of MMP-3 and TIMP-1 in the skin ulcer tissue of diabetic rats.
METHODSThe skin ulcer model was established in diabetic mice. Different compatibility proportions of SHR [the ratio of Shengji Recipe (SJR) to Huayu Recipe (HYR) = 2:1, 1:1, and 1:2, respectively] were used to intervene. The expression of MMP-3 protein in the skin ulcer of diabetic rats was detected by Western blot method,and TIMP-1 protein was detected by immunohistochemical assay.
RESULTSAt each time point, there was no statistical difference in the blood glucose level among groups (P > 0.05). But all of them increased significantly,when compared with those of the normal wound group (P < 0.01). As for the difference between after would area treatment and before would area treatment, better effect was obtained in the SHR No. 3 group and the normal ulcer group than in the diabetic ulcer model group (P < 0.05). Results of Western blot showed that the MMP-3 protein expression was higher in the SHR No. 2 group than in the SHR No.3 group (P < 0.05). Immunohistochemical results showed that TIMP-1 protein expression was lower in the SHR No. 2 group than in the SHR No. 3 group and the diabetic ulcer model group (P < 0.05). TIMP-1 protein expression was higherin the SHR No. 3 group than in the SHR No. 2 group (P < 0.01).
CONCLUSIONUsing SHR No.3 was conducive to the promotion of wound healing in early wound repair stage, and using SHR No. 2 might be conducive to inhibiting the formation of pathological scar.