Effect of Yangjing Zhongyu Decoction on mRNA and protein expression of PCNA, StAR, and FSHR in ovarian granulosa cells cultured by excess androgen.
- Author:
Yan-Hua ZHENG
;
Tao DING
;
Hong-Xia MA
;
Dan-Feng YE
;
Nian-Jun SU
;
Xiao-Ke WU
- Publication Type:Journal Article
- MeSH: Androgens; pharmacology; Animals; Cells, Cultured; Drugs, Chinese Herbal; pharmacology; Female; Follicle Stimulating Hormone; pharmacology; Granulosa Cells; cytology; drug effects; metabolism; Membrane Transport Proteins; genetics; metabolism; Ovarian Follicle; cytology; drug effects; Proliferating Cell Nuclear Antigen; genetics; metabolism; RNA, Messenger; genetics; Receptors, FSH; genetics; metabolism; Swine
- From: Chinese Journal of Integrated Traditional and Western Medicine 2014;34(3):312-316
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of Yangjing Zhongyu Decoction (YZD) on mRNA and protein expression of PCNA, StAR, and FSHR in ovarian granulose cells (GCs) cultured by excess androgen.
METHODSOvarian GCs from porcine follicles were isolated and cultured in vitro. Follicular stimulating hormone (FSH) or YZD was added in the GCs treated by excess testosterone propionate. Totally 48 h later mRNA and protein expression of PCNA, StAR, and FSHR were detected by RT-PCR and Western blot.
RESULTSExcess androgen inhibited mRNA and protein expression of PCNA, StAR, and FSHR of GCs. FSH and YZD could antagonize inhibition of excess androgens, and promote mRNA and protein expression of PCNA, StAR, and FSHR in GCs.
CONCLUSIONYZD could antagonize the inhibition of excess androgen on mRNA and protein expression of PCNA, StAR and FSHR in GCs. Thus, we inferred that YZD could improve the follicle dysplasia by promoting mRNA and protein expression of PCNA, StAR and FSHR in GCs.