Establishment of urinary proteomic map on two-dimensional polyacrylamide gel electrophoresis in children with idiopathic nephrotic syndrome.
- Author:
Juan-Juan DING
1
;
Qing-Nan HE
;
Pin ZHOU
;
Lei ZHANG
;
Zhu-Wen YI
Author Information
- Publication Type:Journal Article
- MeSH: Child; Electrophoresis, Gel, Two-Dimensional; Female; Humans; Male; Nephrotic Syndrome; urine; Proteinuria; urine; Proteomics; methods
- From: Chinese Journal of Contemporary Pediatrics 2007;9(2):122-124
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a urinary proteomic map on two-dimensional polyacrylamide gelelectrophoresis (2-DE) in children with idiopathic nephrotic syndrome (INS).
METHODSThe proteins from INS children were purified by four various means, separated by 2-DEand stained by silver.
RESULTSThe sequential preparation of urinary proteins by acetone precipitation and dislysis, when the sample was 300 microg, resulted in a clear background, well-resolved and reproducible 2-DE urinary protemic map in children with INS.
CONCLUSIONSA steady 2-DE technique for urinary protemic map in children with INS was established, which can be effectively applied in urinary proteomics of the disease.
