Amentoflavone induces apoptosis in SW480 human colorectal cancer cells via regulating β-catenin and caspase-3 expressions.
- Author:
Yu YANG
1
;
Wenjuan XU
;
Kang PENG
;
Xuegang SUN
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Biflavonoids; pharmacology; Caspase 3; metabolism; Cell Line, Tumor; drug effects; Colorectal Neoplasms; pathology; Humans; beta Catenin; metabolism
- From: Journal of Southern Medical University 2014;34(7):1035-1038
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of β-catenin and caspase-3 in amentoflavone-induced apoptosis of human colorectal cancer SW480 cells.
METHODSMTT assay was used to detect the viability of SW480 cells exposed to amentoflavone, and flow cytometry was employed to assess the cell apoptosis. Western blotting was performed to determine the protein expressions of β-catenin and caspase-3 in the exposed cells.
RESULTSAmentoflavone dose-dependently inhibited the viability of SW480 cells, and a high concentration of amentoflavone (150 µmol/L) obviously induced apoptosis of the cells. Amentoflavone exposure caused significantly increased expression of caspase-3 and suppressed β-catenin expression in the cells.
CONCLUSIONAmentoflavone-induced apoptosis in SW480 human colorectal cancer cells is associated with altered expressions of β-catenin and caspase-3.
