Association of human chorionic gonadotropin level in embryo culture media with early embryo development.
- Author:
Haiying WANG
1
;
Renli ZHANG
;
Dong HAN
;
Caixia LIU
;
Jiajie CAI
;
Yanling BI
;
Anmin WEN
;
Song QUAN
Author Information
- Publication Type:Journal Article
- MeSH: Biomarkers; chemistry; Chorionic Gonadotropin; chemistry; Culture Media; chemistry; Embryo Transfer; Embryonic Development; Fertilization in Vitro; Humans
- From: Journal of Southern Medical University 2014;34(7):1039-1047
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the association of human chorionic gonadotropin (HCG) level on day 3 of embryo culture with embryo development.
METHODSSpent culture media were collected from individually cultured embryos on day 3 of in vitro fertilization and embryo transfer (IVF-ET) cycles. HCG concentration in the culture media was measured using an ELISA kit and its association with embryo development was assessed.
RESULTSIn the 163 samples of embryo culture media from 60 patients, HCG was positive in 153 sample (93.8%) with a mean level of 0.85 ± 0.43 mIU/ml. The concentration of hCG in the culture media increased gradually as the number of blastomeres increased (F=2.273, P=0.03), and decreased as the morphological grade of the embryo was lowered (F=3.900, P=0.02).
CONCLUSIONELISA is capable of detecting HCG levels in spent culture media of embryos on day 3 of in vitro culture. The concentration of HCG in spent culture media is positively correlated with the status of early embryo development and implantation rate and thus serves as a useful marker for embryo selection in IVF-ET procedure.
