Changes of platelet mitochondria in rats with tourniquet-induced limb ischemia- reperfusion injury.
- Author:
Jianxing ZHANG
1
;
Hongying LI
;
Guobin ZHOU
;
Qing WANG
;
Zhen LI
Author Information
- Publication Type:Journal Article
- MeSH: Adenosine Triphosphate; metabolism; Animals; Blood Platelets; pathology; Cytochromes c; blood; Hydrogen Peroxide; metabolism; Membrane Potential, Mitochondrial; Mitochondria; pathology; Platelet Count; Rats; Rats, Sprague-Dawley; Reperfusion Injury; Tourniquets
- From: Journal of Southern Medical University 2014;34(7):1075-1078
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the changes of platelet mitochondria in rats with tourniquet-induced limb ischemia-reperfusion (IR) injury.
METHODSThirty SD rats were randomized equally into 5 groups including a control group and 4 limb IR injury groups for blood sampling at 2, 6, 12, or 24 h following IR injury induced by tourniquet on the thighs for 4 h. Platelet was separated from the blood samples for measurement of ATP content, mitochondrial membrane potentials, plasma cytochrome C level, and hydroperoxides.
RESULTSCompared with the control group, the rats with tourniquet-induced limb IR injury showed significantly decreased ATP content, lowered mitochondrial membrane potential, and increased plasma cytochrome C and hydroperoxide levels in the platelets at 2 and 6 h following the injury (P<0.05 or 0.01). These alterations recovered partially but remained significantly different from the control levels at 12 h (P<0.05 or 0.01) until full recovery at 24 h. Limb IR injury did not cause significant variations of the platelet counts.
CONCLUSIONTourniquet-induced limb IR injury can cause mitochondrial damage in the platelets, which occurs mainly in the early stage (6 h) and recovers gradually afterwards without significant impact on platelet counts.
