Effect of bone marrow mesenchymal stem cells on lipopolysacharide-induced secretion of inflammatory cytokines in rat macrophages in vitro.
- Author:
Huanli WANG
1
,
2
;
Bing XIONG
;
Huade CHEN
;
Wen LAI
;
Shaoyi ZHENG
;
Huining BIAN
;
Zuan LIU
;
Zhifeng HUANG
;
Chuanwei SUN
;
Lianghua MA
;
Hanhua LI
;
Lijun WEI
;
Hanxi CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; Coculture Techniques; Enzyme-Linked Immunosorbent Assay; Inflammation; Interleukin-10; secretion; Lipopolysaccharides; Macrophages; secretion; Mesenchymal Stromal Cells; cytology; Rats; Tumor Necrosis Factor-alpha; secretion
- From: Journal of Southern Medical University 2014;34(9):1259-1264
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of bone marrow mesenchymal stem cells (BMSCs) on secretion of inflammatory cytokines in macrophages stimulated by lipopolysacharide (LPS).
METHODSRat BMSCs and macrophages were isolated, cultured, and identified. The BMSCs and macrophages, cultured alone or in co-culture, were treated with LPS or PBS or without treatment and tested for interleukin-10 (IL-10) and tumor necrosis factor-α (TNF-α) concentrations in the supernatants at 1, 3, 5, 7, 12, and 24 h after the treatment using enzyme-linked immunosorbent assay.
RESULTSExposure to LPS caused significantly increased IL-10 and TNF-α concentrations in the supernatant of cultured macrophages but not in BMSC culture. Macrophages co-cultured with BMSCs showed significantly lowered IL-10 and TNF-α secretions in response to LPS exposure as compared with the macrophages cultured alone.
CONCLUSIONBMSCs can reduce LPS-induced secretion of inflammatory cytokines by the macrophages to ameliorate inflammatory reactions.
