Protective effect of rutin against lipopolysaccharide-induced acute lung injury in mice.
- Author:
Longhua FENG
1
;
Daoxin WANG
;
Jing HE
;
Di QI
Author Information
- Publication Type:Journal Article
- MeSH: Acute Lung Injury; chemically induced; drug therapy; Animals; Bronchoalveolar Lavage Fluid; Epithelial Sodium Channels; metabolism; Interleukin-1beta; metabolism; Lipopolysaccharides; Lung; pathology; Mice; Mice, Inbred C57BL; RNA, Messenger; Rutin; pharmacology; Tumor Necrosis Factor-alpha; metabolism
- From: Journal of Southern Medical University 2014;34(9):1282-1285
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the protective effect of rutin against acute lung injury induced by lipopolysaccharide (LPS).
METHODSThirty C57BL/6 mice were randomly divided into control group, LPS-induced acute lung injury model group and treatment (LPS+Rutin) group. The pathological changes of the lung tissue were observed microscopically on paraffin sections with HE staining, and the lung wet/dry weight ratio was measured. The levels of TNF-α and IL-1β in the bronchoalveolar lavage fluid (BALF) were measured with ELISA, and the expressions of α-ENaC were detected with RT-PCR and Western blotting.
RESULTSPathological examination of the lung tissue revealed distinct inflammation, congestion and edema in the model group. The mice in the treatment group showed significantly milder lung injuries than those in the model group. Compared with the control group, the model group showed significantly increased lung wet/dry ratio and contents of TNF-α and IL-1β in BALF but lowered expressions of α-ENaC mRNA and protein. Compared with the model group, rutin treatment significantly decreased the lung wet/dry ratio and TNF-α and IL-1β levels in the BALF and increased the expressions of α-ENaC mRNA and protein.
CONCLUSIONRutin can inhibit the pulmonary inflammation and increase the expression of alveolar epithelial sodium channel protein to alleviate LPS-induced acute lung injury in mice.
