¹H NMR metabonomics study of pancreatic extracts from insulin-resistant rats induced by fructose feeding.
- Author:
Linlin WANG
1
;
Lingyun ZHENG
;
Lei ZHANG
;
Ali CHEN
;
Cuihuan QIU
;
Jingfen XU
;
Yongxia YANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Fructose; Insulin Resistance; Metabolomics; Pancreatic Extracts; chemistry; Principal Component Analysis; Proton Magnetic Resonance Spectroscopy; Rats; Rats, Wistar
- From: Journal of Southern Medical University 2014;34(9):1301-1304
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the metabolic changes of pancreatic extracts from insulin-resistant rats induced by fructose feeding using nuclear magnetic resonance ¹H spectroscopy (¹H NMR).
METHODSSixteen Wistar rats were divided equally into control group and model group and given water and 10% fructose water for 8 weeks, respectively. The pancreatic tissues were then obtained for H NMR spectra analysis and principal component analysis (PCA).
RESULTSCompared with the control rats, the rats in the model group showed significantly increased creatine, betaine/TMAO, taurine, glycine and myo-inositol and decreased levels of lipids, lactate, glutamate, choline and GPC/PC.
CONCLUSION¹H NMR and pattern recognition can define the metabolic characteristics of the pancreatic tissue extracts from insulin-resistant rats and provide reliable metabolic evidence for studying the mechanisms of insulin resistance at the molecular level.
