Cytotoxicity of T cells transduced with WT1 peptide-specific T-cell receptor gene against human lung cancer cells in vitro.
- Author:
Jun AN
1
;
Song-Wang CAI
;
Yun LI
;
Junhang ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: CD8-Positive T-Lymphocytes; cytology; Cell Line, Tumor; Genes, T-Cell Receptor; Humans; Immunotherapy, Adoptive; Lung Neoplasms; pathology; Peptides; Receptors, Antigen, T-Cell, alpha-beta; genetics; Retroviridae; T-Lymphocytes, Cytotoxic; cytology; Transduction, Genetic; WT1 Proteins; genetics
- From: Journal of Southern Medical University 2014;34(9):1319-1323
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the cytotoxicity of normal CD8(+) T lymphocytes retrovirally transduced with WT1 peptide-specific T-cell receptor (TCR) genes against human lung cancer cells.
METHODSHLA-A*2402-restricted and WT1 peptide-specific TCR-α/β genes were cloned from a cytotoxic T lymphocyte clone and inserted into a retroviral TCR expression vector. The cytotoxicity of normal peripheral CD8⁺ T cells transduced with the WT1-TCR genes against human lung cancer cells was evaluated using a standard ⁵¹Cr release assay.
RESULTSThe WT1-TCR gene-modified T cells recognized the peptide-pulsed target cells but not the non-pulsed cells. TCR-redirected CD8⁺ T cells lysed WT1-overexpressing human lung cancer cells in an HLA-A*2402-restricted manner, but did not kill normal cells positively expressing HLA-A*2402.
CONCLUSIONThese data demonstrate the feasibility of adoptive immunotherapy with TCR-redirected T cell for the treatment of lung cancer.
