miR-128a is up-regulated in hepatocellular carcinoma and promotes tumor cell proliferation by targeting RND3.
- Author:
Xuejun GUO
1
;
Chuanhui CAO
;
Jingyuan SUN
;
Dongyan ZHANG
;
Li LIU
;
Dehua WU
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Hepatocellular; metabolism; Cell Proliferation; Cell Survival; Down-Regulation; Humans; Liver Neoplasms; metabolism; MicroRNAs; genetics; metabolism; RNA, Messenger; Transcriptional Activation; Tumor Cells, Cultured; Up-Regulation; rho GTP-Binding Proteins; metabolism
- From: Journal of Southern Medical University 2014;34(10):1408-1413
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of microRNA-128a (miR-128a) and its role in hepatocellular carcinoma (HCC).
METHODSNineteen pairs of fresh surgical specimens of HCC and adjacent tissues were examined for miR-128a expression using qRT-PCR. A miR-128a mimics or inhibitor was transfected into HCC cells, and the cell viability was analyzed by MTT assay. RND3, one of the potential targets of miR-128a, was predicted by bioinformatics software and demonstrated by dual luciferase reporter assay. The expression of RND3 after transfection was detected using qRT-PCR and Western blotting, and the cell cycle-related proteins were determined with Western blotting.
RESULTSThe expression of miR-128a were significantly up-regulated in HCC tissues as compared to the adjacent tissues (P<0.05). In cultured HCC cells, miR-128a promoted the cell proliferation and resulted in down-regulated RND3 mRNA and protein expressions by targeting RND3' 3'UTR (P<0.05) and also in the down-regulation of cyclin B1, cyclin D1 and CDK4 protein expressions.
CONCLUSIONmiR-128a is up-regulated in HCC and promotes HCC cell proliferation by targeting RND3.
