Study of the anti-endotoxin effect of beta-1, 2, 3, 4, 6-penta-O-galloyl-D-glucopyranose in vitro.
- Author:
Gen-fa LV
1
;
Guang-xia XIAO
;
Guo WEI
;
Yi-bin GUO
;
Jiang ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Biosensing Techniques; Cells, Cultured; Dose-Response Relationship, Drug; Drug Antagonism; Endotoxins; pharmacology; Humans; Hydrolyzable Tannins; pharmacology; In Vitro Techniques; Interleukin-6; metabolism; Leukocytes, Mononuclear; metabolism; Lipid A; pharmacology; Tumor Necrosis Factor-alpha; metabolism
- From: Chinese Journal of Burns 2005;21(4):282-284
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the anti-endotoxin effect of beta-1, 2, 3, 4, 6-penta-O-galloyl-D-glucopyranose (PGG) in vitro.
METHODSThe affinity of PGG with lipid A was determined with biosensor technology, and the endotoxin-neutralizing effect was assayed with LAL. Human peripheral blood mononuclear cells (hPBMC) were separated from healthy donors and cultured in vitro. The effect of different concentrations of PGG on the release of TNF-alpha and hIL-6 from LPS-stimulated hPBMC were measured by ELISA method.
RESULTSLipid A was combined with different concentrations of PGG. The combination speed was shortened with the increase in PGG concentration. The KD value between PGG and Lipid A was 5.2 x 10(-7) mol/L. The release of TNF-alpha and IL-6 of hPBMC under LPS stimulation (958 +/- 234 ng/L vs 1 351 +/- 99 ng/L) was obviously inhibited by PGG in the concentration of higher than 20 mg/L compared with that without PGG treatment (1 788 +/- 171 ng/L vs 1 965 +/- 232 ng/L, P < 0.05).
CONCLUSIONPGG show an anti-endotoxin effect in vitro, which may be associated with its ability to combine and neutralize endotoxin.
