The expression of platelet-derived growth factor-A and its receptor in acute radiation-induced skin ulcer in rats.
- Author:
Wei-hong CAO
1
;
Jia-ke CHAI
;
Zhi-xiang YANG
;
De-wen WANG
;
Zhi-yong SHENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Female; Gamma Rays; adverse effects; Platelet-Derived Growth Factor; biosynthesis; Radiation Injuries, Experimental; complications; metabolism; Rats; Rats, Wistar; Receptor, Platelet-Derived Growth Factor alpha; biosynthesis; Skin Ulcer; etiology; metabolism; Wound Healing
- From: Chinese Journal of Burns 2005;21(5):359-362
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the dynamic expression of platelet-derived growth factor-A (PDGF-A) and its receptor alpha (PDGFR-alpha) in different acute radiation-induced skin ulcers, and to explore the underlying mechanism involved in retarded healing of the ulcer.
METHODSThe model of acute radiation-induced skin ulcers in rats was replicated with 50 Gy 60Co gamma rays to the skin (radiation group, R, n = 55), rats with full - thickness skin excision wounds as control group (T, n = 55), and 5 normal rats to serve as normal control (NC) group. The expression of PDGF-A and PDGFR-alpha protein and PDGF-A mRNA was respectively assessed by means of histochemistry and in situ RT-PCR.
RESULTSNo PDGF-A expression was identified in the rat skin in NC group. The expression of PDGF-A and PDGFR were reduced in R group during inflammatory responsive and granulation formation periods (14 - 28 days after radiation, the IA value of PDGF-A varied from 14.0 +/- 1.2 to 20.3 +/- 1.2 compared with that in T group in which the IA value of PDGF-A at the same period (3 - 9 days after injury) varied from 20.0 +/- 1.6 to 28.3 +/- 1.0, and reduced gradually during scar formation period (55 days after radiation).
CONCLUSIONThe reduction of PDGF-A and PDGFR-expression may be partially involved in the mechanism of retarded healing of acute radiation-induced skin ulcers.
