Seasonal abundance and potential of Japanese encephalitis virus infection in mosquitoes at the nesting colony of ardeid birds, Thailand.

Tanasak Changbunjong; Thekhawet Weluwanarak; Namaoy Taowan; Parut Suksai; Tatiyanuch Chamsai; Poonyapat Sedwisai

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Seasonal abundance and potential of Japanese encephalitis virus infection in mosquitoes at the nesting colony of ardeid birds, Thailand.

Author: Tanasak CHANGBUNJONG ¹ ; Thekhawet WELUWANARAK ; Namaoy TAOWAN ; Parut SUKSAI ; Tatiyanuch CHAMSAI ; Poonyapat SEDWISAI
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1. The Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals, Faculty of Veterinary Science, Mahidol University, Nakorn Pathom, Thailand.
Publication Type:Journal Article
Keywords: Abundance; Ardeid bird; Japanese encephalitis virus; Mosquito; Vector
MeSH: Animals; Bird Diseases; epidemiology; virology; Birds; Culicidae; physiology; virology; Encephalitis Virus, Japanese; isolation & purification; Encephalitis, Japanese; epidemiology; veterinary; virology; Population Dynamics; Reverse Transcriptase Polymerase Chain Reaction; veterinary; Seasons; Thailand; epidemiology; Virus Cultivation; veterinary
From:Asian Pacific Journal of Tropical Biomedicine 2013;3(3):207-210
CountryChina
Language:English
Abstract:
OBJECTIVETo investigate the abundance and seasonal dynamics of mosquitoes, and to detect Japanese encephalitis virus (JEV) in these mosquitoes at the nesting colony of ardeid birds.
METHODSMosquitoes were collected bimonthly from July 2009 to May 2010 by Centers for Disease Control. Light traps and dry ice, as a source of CO2, were employed to attract mosquitoes. Mosquitoes were first identified, pooled into groups of upto 50 mosquitoes by species, and tested for JEV infection by viral isolation and reverse transcriptase polymerase chain reaction.
RESULTSA total of 20 370 mosquitoes comprising 14 species in five genera were collected. The five most abundant mosquito species collected were Culex tritaeniorhynchus (95.46%), Culex vishnui (2.68%), Culex gelidus (0.72%), Anopheles peditaeniatus (0.58%) and Culex quinquefasciatus (0.22%). Mosquito peak densities were observed in July. All of 416 mosquito pools were negative for JEV.
CONCLUSIONSThis study provides new information about mosquito species and status of JEV infection in mosquitoes in Thailand. Further study should be done to continue a close survey for the presence of this virus in the ardeid birds.