Carbohydrase inhibition and anti-cancerous and free radical scavenging properties along with DNA and protein protection ability of methanolic root extracts of Rumex crispus.
- Author:
Supriya SHIWANI
1
;
Naresh Kumar SINGH
;
Myeong Hyeon WANG
Author Information
- Publication Type:Original Article
- Keywords: Root of Rumex crispus; methanolic extract; antioxidative; carbohydrase inhibitor; anti-cancerous
- MeSH: alpha-Glucosidases; Amylases; Biphenyl Compounds; DNA; DNA Damage; Glycoside Hydrolases; HT29 Cells; Humans; Methanol; Phenol; Picrates; Power (Psychology); Rumex; Vacuum
- From:Nutrition Research and Practice 2012;6(5):389-395
- CountryRepublic of Korea
- Language:English
- Abstract: The study elucidated carbohydrase inhibition, anti-cancerous, free radical scavenging properties and also investigated the DNA and protein protection abilities of methanolic root extract of Rumex crispus (RERC). For this purpose, pulverized roots of Rumex crispus was extracted in methanol (80% and absolute conc.) for 3 hrs for 60degrees C and filtered and evaporated with vacuum rotary evaporator. RERC showed high phenolic content (211 microg/GAE equivalent) and strong 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging (IC50 = 42.86 (absolute methanol) and 36.91 microg/mL (80% methanolic extract)) and reduced power ability. Furthermore, RERC exhibited significant protective ability in H2O2/Fe3+/ascorbic acid-induced protein or DNA damage and percentage inhibition of the HT-29 cell growth rate following 80% methanolic RERC exposure at 400 microg/mL was observed to be highest (10.2% +/- 1.03). Moreover, methanolic RERC inhibited alpha-glucosidase and amylase effectively and significantly (P < 0.05). Conclusively, RERC could be considered as potent carbohydrase inhibitor, anti-cancerous and anti-oxidant.
