Optimized mixture of hops rho iso-alpha acids-rich extract and acacia proanthocyanidins-rich extract reduces insulin resistance in 3T3-L1 adipocytes and improves glucose and insulin control in db/db mice.
- Author:
Matthew L TRIPP
1
;
Gary DARLAND
;
Veera Reddy KONDA
;
Linda M PACIORETTY
;
Jyh Lurn CHANG
;
Jeffrey S BLAND
;
John G BABISH
Author Information
- Publication Type:In Vitro ; Original Article
- Keywords: Humulus lupulus; Acacia nilotica; phytochemicals; metabolic syndrome; anti-inflammatory
- MeSH: Acacia; Adipocytes; Animals; Aspirin; Chromans; Glucose; Humulus; Insulin; Insulin Resistance; Interleukin-6; Lipolysis; Metformin; Mice; Thiazolidinediones
- From:Nutrition Research and Practice 2012;6(5):405-413
- CountryRepublic of Korea
- Language:English
- Abstract: Rho iso-alpha acids-rich extract (RIAA) from Humulus lupulus (hops) and proanthocyanidins-rich extracts (PAC) from Acacia nilotica exert anti-inflammatory and anti-diabetic activity in vitro and in vivo. We hypothesized that a combination of these two extracts would exert enhanced effects in vitro on inflammatory markers and insulin signaling, and on nonfasting glucose and insulin in db/db mice. Over 49 tested combinations, RIAA:PAC at 5:1 (6.25 microg/mL) exhibited the greatest reductions in TNFalpha-stimulated lipolysis and IL-6 release in 3T3-L1 adipocytes, comparable to 5 microg/mL troglitazone. Pretreatment of 3T3-L1 adipocytes with this combination (5 microg/mL) also led to a 3-fold increase in insulin-stimulated glucose uptake that was comparable to 5 microg/mL pioglitazone or 901 microg/mL aspirin. Finally, db/db mice fed with RIAA:PAC at 5:1 (100 mg/kg) for 7 days resulted in 22% decrease in nonfasting glucose and 19% decrease in insulin that was comparable to 0.5 mg/kg rosiglitazone and better than 100 mg/kg metformin. RIAA:PAC mixture may have the potential to be an alternative when conventional therapy is undesirable or ineffective, and future research exploring its long-term clinical application is warranted.
