Influence of gastric bypass surgery on hepatic gluconeogenesis in type 2 diabetic Goto Kakizaki rats.
- Author:
Song WU
1
;
Jun-cheng TANG
;
Hong ZOU
;
Hong-tao YAN
;
Tao CHEN
;
Dong-xuan LI
;
Li-jun TANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blood Glucose; analysis; Diabetes Mellitus, Experimental; metabolism; surgery; Diabetes Mellitus, Type 2; metabolism; surgery; Gastric Bypass; Gluconeogenesis; Glucose-6-Phosphatase; metabolism; Glycated Hemoglobin A; metabolism; Intracellular Signaling Peptides and Proteins; metabolism; Liver; enzymology; Male; Phosphoenolpyruvate Carboxykinase (GTP); metabolism; Rats
- From: Chinese Journal of Gastrointestinal Surgery 2012;15(11):1142-1145
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the influence and significance of gastric bypass surgery on hepatic gluconeogenesis in type 2 diabetic Goto Kakizaki(GK) rats.
METHODSForty GK rats were randomly divided into Roux-en-Y gastric bypass group(group A) and sham operation group(group B). Differences in glucose tolerance experiment(OGTT) at preoperative and postoperative 1, 2 and 4 weeks were compared and weight was recorded. Glycated hemoglobin levels were measured preoperatively and 4 weeks postoperatively. The animals were sacrificed 4 weeks after surgery and liver tissues were harvested to detect the relative expression of mRNA and protein of glucose 6 phosphatase(G-6-P) and phosphoenol pyruvate kinase(PEPCK) with RT-PCR and Western blot.
RESULTSFasting blood glucose levels were 6.5, 4.9, and 4.7 mmol/L in group A, and were 10.3, 10.4, and 12.5 mmol/L in group B, and the differences between two groups were statistically significant(P<0.05). The blood glucose level at 2 h after stomach lavage were 8.3, 6.4 and 5.5 mmol/L in group A, and were 21.4, 23.8 and 24.7 mmol/L in group B at postoperative 1, 2, 4 weeks, and the differences between two groups were statistically significant(P<0.05). The glycosylated hemoglobin at postoperative 4 weeks was(6.8±1.0)%, significantly lower than that in group B[(7.9±0.8)%, P<0.05]. Hepatic G-6-P and PEPCK mRNA relative expression at postoperative 4 weeks was reduced by 21.0% and 25.9% respectively as compared to group B, and the protein expression reduced as well. Immunohistochemistry showed that hepatic glycogen sedimentary in group A increased significantly.
CONCLUSIONThe relative mRNA and protein level of key enzymes of hepatic gluconeogenesis are significantly decreased after Roux-en-Y gastric bypass surgery and hepatic gluconeogenesis is reduced, which may be a potential mechanism of the decrease of blood glucose.