Expression of hMLH1 in rectal intraepithelial neoplasm and early rectal carcinoma.
- Author:
Zhi-xuan XUAN
1
;
Guo-le LIN
;
Xin-ming YU
;
Hui-zhong QIU
Author Information
- Publication Type:Journal Article
- MeSH: Adaptor Proteins, Signal Transducing; genetics; metabolism; Adult; Aged; Aged, 80 and over; Carcinoma in Situ; diagnosis; metabolism; Early Detection of Cancer; Female; Humans; Male; Middle Aged; MutL Protein Homolog 1; Nuclear Proteins; genetics; metabolism; Rectal Neoplasms; diagnosis; metabolism
- From: Chinese Journal of Gastrointestinal Surgery 2012;15(11):1162-1165
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore whether the abnormality of hMLH1 gene may be an early event of carcinogenesis in rectal carcinoma, and to evaluate the diagnostic value in differentiation between intraepithelial neoplasm and early stage of colorectal carcinoma.
METHODSThe expression of hMLH1 protein in 28 cases with early invasive rectal carcinoma(EIRC), 36 cases with rectal intraepithelial neoplasm(RIEN), and 30 cases with normal rectal mucosa(NRM) which were collected through surgical operations were detected by PV-9000 immunohistochemical method.
RESULTSThe positive expression rates of hMLH1 protein were 100%(30/30), 77.8%(28/36), and 39.3%(11/28) in NRM, RIEN, and EIRC respectively. The difference was statistically significant between RIEN and EIRC(P=0.002), and the difference was also statistically significant between RIEN and NRM(P=0.006). The positive expression of hMLH1 was not related to age, gender, tumor maximum diameter, dysplasia, tumor types, and distance from the anal verge in RIEV group(P>0.05). In EIRC group, hMLH1 was associated with tumor differentiation(P<0.05).
CONCLUSIONhMLH1 gene deletion may be an early event during carcinogenesis of rectal carcinoma, which may be useful in differentiation of intraepithelial neoplasm from early rectal carcinoma.
