Effect of retinoic acid on the development of B cells from lymph nodes of young children and the pathway of the effect.
- Author:
Dong WEI
1
;
Yi YANG
;
Wei-ping WANG
Author Information
- Publication Type:Journal Article
- MeSH: B-Lymphocytes; drug effects; physiology; Cells, Cultured; Child, Preschool; Female; Humans; Infant; Lymph Nodes; drug effects; Lymphocyte Activation; drug effects; Male; Receptors, Retinoic Acid; genetics; Retinoic Acid Receptor alpha; Tretinoin; pharmacology
- From: Chinese Journal of Pediatrics 2005;43(5):360-363
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of retinoic acid on the differentiation and maturation of B cells from lymph node of children, and relational changes of the expression levels of retinoic acid receptor genes.
METHODSTwenty-four patients with digestive tract malformation underwent surgical operation in the surgical ward of our hospital. They were divided into 3 groups according to age: < 1 yr, 1 - 3 yr, -5 yr, 8 cases in each age group. The lymph nodes in the margin of excised tissues were obtained. The cells separated from lymph nodes were cultured in vitro. The cells were divided into 5 groups: Retinoic acid (RA), RA plus Ro41-5253, RA receptor antagonist (RA+Ro), lipopolysaccharide (LPS), lipopolysaccharide plus RA (LPS+RA) and control, and were subjected to the corresponding treatments. After 24 h and 48 h of cell culture, the surface markers on the B cells were detected by flow cytometer to observe the maturation and activation of B cells. The expression levels of retinoic acid receptor genes were quantitatively analyzed by RT- fluorescent quantitative PCR.
RESULTSAfter culture, in < 1 yr group, the number of the mature B cells (IgM(+)IgD(+)CD25(-)) in the RA group was significantly higher than that in the control (24 h: 23% +/- 5% vs. 17% +/- 3%; 48 h: 28% +/- 6% vs. 22% +/- 4%) (P < 0.05), and that in the RA + RO group was not significantly different from that in the control (24 h: 16% +/- 4%; 48 h: 20% +/- 9%) (P > 0.05). The number of activated B cells (IgM(+)CD25(+)) in the LPS + RA group was obviously higher than that in the LPS group (24 h: 82% +/- 10% vs. 76% +/- 8%; 48 h: 83% +/- 8% vs. 78% +/- 10%)(P < 0.05). The level of RARalpha gene expression of B cells (lg copies/50 ng RNA) in the RA group was significantly higher than that in the control (24 h: 7.03 +/- 1.36 vs. 5.79 +/- 2.05; 48 h: 7.91 +/- 1.60 vs. 6.21 +/- 1.88) (P < 0.05), and that in the LPS + RA group was significantly higher than that in the LPS group (24 h: 7.29 +/- 1.53 vs. 5.98 +/- 1.48; 48 h: 7.83 +/- 1.66 vs. 5.79 +/- 2.36)(P < 0.05). In 1 - 3 yr group the changes of maturation and activation of B cells in the lymph nodes were the same as the < 1 yr group. In -5 yr group, such changes were not significant.
CONCLUSIONRA can promote the development of B cell from lymph node in vitro culture in
