Effect of polysaccharide from Spirulina platensis on hematopoietic cells proliferation, apoptosis and Bcl-2 expression in mice bearing tumor treated with chemotherapy.
- Author:
Xiao-mei LIU
1
;
Hong-quan ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antineoplastic Agents, Alkylating; therapeutic use; Apoptosis; Bacterial Proteins; chemistry; Cell Division; drug effects; Cyclophosphamide; therapeutic use; Female; Hematopoietic Stem Cells; drug effects; pathology; Liver Neoplasms, Experimental; drug therapy; metabolism; pathology; Male; Mice; Mice, Inbred ICR; Polysaccharides; isolation & purification; pharmacology; Proto-Oncogene Proteins c-bcl-2; metabolism; Random Allocation; Spirulina
- From: Acta Pharmaceutica Sinica 2002;37(8):616-620
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo evaluate the effect of polysaccharide from Spirulina platensis (PSP) on hematopoietic cell proliferation, apoptosis and Bcl-2 expression in mice bearing tumor treated with chemotherapy.
METHODSThe model of chemotherapy for transplant solid tumor in mice was established. The hematopoietic cell proliferation, apoptosis, Bcl2 expression and related cytokines were assayed by the technique of culture of hematopoietic progenitor cell, fluoromicroscope and light microscope, immunohistochemical method, and double antibody sandwich ELISA.
RESULTSPSP significantly ameliorated CFU-GM proliferation inhibition and hematopietic cells apoptosis induced by CTX. Moreover, PSP evidently increased the content of IL-1, IL-3, GM-CSF and TNF-alpha in serum and Bcl-2 expression of hematopoietic cells.
CONCLUSIONPSP indirectly upregulated Bcl-2 expression of hematopoietic cells by promoting endogenous cytokines secretion which may be one of the mechanisms, by which PSP enhanced hematopoietic cell proliferation and inhibited its apoptosis in mice bearing tumor treated with chemotherapy.
