The enhancing effect of electroporation and iontophoresis on the permeation of insulin through human skin.
- Author:
Yan PAN
1
;
Hui-ying ZHAO
;
Jun-min ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Electroporation; methods; Humans; In Vitro Techniques; Insulin; administration & dosage; pharmacokinetics; Iontophoresis; methods; Permeability; Skin; metabolism; Skin Absorption
- From: Acta Pharmaceutica Sinica 2002;37(8):649-652
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the enhancing effect of electroporation and iontophoresis on the permeation of insulin through human cadaver skin in vitro.
METHODSUsing side by side two-chamber diffusion cells, the flux of insulin achieved with iontophoresis and electrophoration were compared.
RESULTSThe application of high-voltage pulse combined with iontophoresis resulted in higher flux transdermal permeation of insulin than either one technique alone (P < 0.05). Pulsing at a higher voltage increased the flux of insulin more dramatically than pulsing at a lower voltage (P < 0.01). The transdermal transport of insulin by 90 pulse of 500 V (exponential pulse generater, pulse time: 20-24 ms, pulse frequency: 3 pulse.min-1) followed by iontophoresis led to a quick input and a high steady flux.
CONCLUSIONElectroporation combined with iontophoresis can enhance the permeation of insulin significantly.
