Study on metabolites on aconitine in rabbit urine.
- Author:
Ying SUN
1
;
Hong-gui ZHANG
;
Xiang-guo SHI
;
Ming-yu DUAN
;
Da-fang ZHONG
Author Information
- Publication Type:Journal Article
- MeSH: Aconitine; analogs & derivatives; metabolism; urine; Alkaloids; urine; Animals; Chromatography, High Pressure Liquid; Male; Rabbits; Spectrometry, Mass, Electrospray Ionization
- From: Acta Pharmaceutica Sinica 2002;37(10):781-783
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo identify the main metabolites of aconitine in the urine of rabbits.
METHODSAfter oral administration of aconitine (5 mg.kg-1), the urine of male rabbits was collected and extracted by solid phase extraction and analyzed by liquid chromatography-ion trap mass spectrometry.
RESULTSAconitine and 4 metabolites were found in the rabbit urine. Their protonated molecular ions at m/z 632, m/z 604, m/z 590, m/z 500 and multistage fragment ions with neutral loss of 60 u, 32 u, 28 u and 18 u were monitored. Their relative concentration were M1 > Aconitine > M4 > M2 > M3.
CONCLUSIONThe metabolites M1-M4 were deduced as 16-O-demethylaconitine, benzoylaconine, 16-O-demethylbenzoylaconine and aconine, respectively.
