Determination of six effective components in Rheum by cyclodextrin modified micellar electrokinetic chromatography.
- Author:
Xiao-yu SHANG
1
;
Zhuo-bin YUAN
Author Information
- Publication Type:Journal Article
- MeSH: Anthraquinones; analysis; Chromatography, Micellar Electrokinetic Capillary; methods; Cyclodextrins; chemistry; Emodin; analogs & derivatives; analysis; Plants, Medicinal; chemistry; Quality Control; Rheum; chemistry; Stilbenes; analysis
- From: Acta Pharmaceutica Sinica 2002;37(10):798-801
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo determine six effective components (aloe-emodin, rhein, emodin, rhaponticin, physcion and chrysophanol) in Rheum.
METHODSUsing buffer solution containing 20 mmol.L-1 borax, 20 mmol.L-1 sodium deoxycholate (SDC), 20 mmol.L-1 sodium taurocholate (STC), 15 mmol.L-1 beta-cyclodextrin (beta-CD) and O-phthalic acid as the internal standard, the six components were determined by cyclodextrin modified micellar electrokinetic chromatography.
RESULTSIn less than 25 min, aloe-emodin, rhein, emodin, rhaponticin, physion and chrysophanol were separated. The separation conditions were optimized by adjusting buffer pH, concentrations of SDC, STC and beta-CD. The linearity ranges of aloe-emodin, rhein, emodin, rhaponticin, physcion and chrysophanol were 4-34, 5-40, 4-60, 5-80, 6-90 and 5-85 micrograms.mL-1 respectively. Relative standard deviation (RSD) of the method was less than 2.2%. The recoveries of aloe-emodin, rhein, emodin, rhaponticin, physcion and chrysophanol were 100.0%, 98.3%, 100.4%, 94.6%. 95.2% and 93.8% respectively. Raw Rheum, Mongolian Rheum and Rheum tanguticum samples were analyzed.
CONCLUSIONThis method can be an effective one for identification of Rhubarb.
