Studies on evodiamine induced HeLa cell apoptosis.
- Author:
Xiao-fang FEI
1
;
Ben-xiang WANG
;
Takashi IKEJIMA
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents, Phytogenic; pharmacology; Apoptosis; Caspase 3; Caspases; metabolism; Evodia; chemistry; Fruit; chemistry; HeLa Cells; Humans; Plant Extracts; isolation & purification; pharmacology; Plants, Medicinal; chemistry; Quinazolines; isolation & purification; pharmacology; Signal Transduction; drug effects
- From: Acta Pharmaceutica Sinica 2002;37(9):673-676
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the mechanism of evodiamine-induced growth inhibition of HeLa cells.
METHODSHeLa cells viability and the effect of caspase inhibitors on evodiamine-induced apoptosis were measured by crystal violet assay. Changes in cellular morphology were observed by phase-contrast microscopy. Apoptosis-specific nucleosomal DNA fragmentation was assayed by agarose gel electrophoresis.
RESULTSEvodiamine was found to inhibit HeLa cell growth in dose- and time-dependent manners. Caspase-3 inhibitor, z-Asp-Glu-Val-Asp-fmk (z-DEVD-fmk) was shown to partially inhibit evodiamine-induced apoptosis. However, caspase-1 inhibitor, Ac-Tyr-Val-Ala-Asp-chloromethyl-ketone (Ac-YVAD-cmk), did not antagonize evodiamine induced cell death.
CONCLUSIONEvodiamine suppresses the growth of HeLa cells in vitro by apoptosis. Evodiamine-induced apoptosis is partially dependent on caspase-3 pathway in HeLa cells. Other apoptotic pathways might be also related to the induction of apoptosis by evodiamine.
