Antagonistic effect of hydroxysafflor yellow A on the platelet activating factor receptor.
- Author:
Bao-xia ZANG
1
;
Ming JIN
;
Nan SI
;
Yan ZHANG
;
Wei WU
;
Yong-zhe PIAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Carthamus; chemistry; Cell Aggregation; drug effects; Chalcone; analogs & derivatives; isolation & purification; pharmacology; In Vitro Techniques; Male; Neutrophils; drug effects; Plants, Medicinal; chemistry; Platelet Aggregation; drug effects; Platelet Membrane Glycoproteins; antagonists & inhibitors; Quinones; isolation & purification; pharmacology; Rabbits; Receptors, G-Protein-Coupled; antagonists & inhibitors
- From: Acta Pharmaceutica Sinica 2002;37(9):696-699
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo observe the antagonistic effect of hydroxysafflor yellow A (HSYA) on the platelet activating factor (PAF).
METHODSWashed rabbit platelet (WRP) aggregation and rabbit polymorphonuclear leukocytes (PMNs) aggregation induced by PAF were observed by turbidimetric assay in vitro. The PAF receptor antagonistic effect of HSYA was investigated by radio ligand binding assay (RLBA).
RESULTSIn RLBA the specific binding inhibition effect of HSYA was found to be concentration-dependent in three different [3H]PAF concentrations. In the experiments, WRP aggregation and rabbit PMNs aggregation induced by PAF (9.55 x 10(-10), 9.55 x 10(-6) mol.L-1) were both inhibited by HSYA in a concentration-dependent manner in vitro. The IC50 of HSYA to inhibit WRP and rabbit PMNs aggregation was 0.99 and 0.70 mmol.L-1, respectively.
CONCLUSIONThe PAF receptor binding can be antagonized by HSYA.
