Determination of irisquinone by single sweep oscillopolarography.
- Author:
Ping HE
1
;
Jun-feng SONG
;
Wei GUO
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents, Phytogenic; administration & dosage; analysis; chemistry; Benzoquinones; administration & dosage; analysis; chemistry; isolation & purification; Capsules; Electrochemistry; Iris; chemistry; Plants, Medicinal; chemistry; Polarography; Seeds; chemistry
- From: Acta Pharmaceutica Sinica 2002;37(3):207-209
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo propose a polarographic method for the determination of irisquinone.
METHODSA reduction wave of irisquinone was recorded by single sweep oscillopolarography.
RESULTSIn 8.0 x 10(-3) mol.L-1 Na2B4O7-1.6 x 10(-2) mol.L-1 KH2PO4 (pH 7.7) supporting electrolyte, a redution wave of irisquinone with peak potential -1.23 V (vs SCE) achieved high sensitivity. The 2nd-order derivative peak current of the reduction wave was proportional to irisquinone concentration in the range of 1.5 x 10(-7)-5.2 x 10(-6) mol.L-1 (gamma = 0.9992, n = 9). The detection limit was 6.0 x 10(-8) mol.L-1. Relative standard deviation (RSD) was 0.87% by performing 13 independent measurements on 2.0 x 10(-6) mol.L-1 irisquinone.
CONCLUSIONThe proposed method was sensitive, simple, rapid, and can be applied to the determination of irisquinone in raw medicine and capsule.
