Effect of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry on identifing biomarkers of endometriosis.
- Author:
Hong ZHANG
1
;
Jie FENG
;
Xiao-hong CHANG
;
Zhong-xing LI
;
Xiao-yi WU
;
Heng CUI
Author Information
- Publication Type:Journal Article
- MeSH: Biomarkers; blood; Endometriosis; blood; diagnosis; Female; Humans; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; methods
- From: Chinese Medical Journal 2009;122(4):373-376
- CountryChina
- Language:English
-
Abstract:
BACKGROUNDEndometriosis is a common gynecological disease. This study aimed to screen proteins that were expressed differently in patients with endometriosis versus normal controls using proteomic techniques, surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS).
METHODSProtein chip SELDI-TOF-MS combines the advantages of microarray and mass spectrometry, and can screen latent markers in sera of patients with endometriosis. Serum samples from patients and normal volunteers were analyzed by SELDI-TOF-MS.
RESULTSAfter comparing the serum protein spectra of 36 patients with 24 normal controls, 24 differently expressed potential biomarkers (P < 0.01) were identified. Using Biomarker Pattern software, we established a tree model of the 60 serum protein spectra. When using the three biomarkers to classify the samples, the sensitivity for diagnosing endometriosis was 91.7%, specificity was 95.8%, and coincidence rate was 93.3%. Then we used serum samples from 12 patients and 8 normal controls to validate the tree model and report the sensitivity for diagnosing endometriosis was 91.7%, specificity was 75%, and coincidence rate was 85%.
CONCLUSIONSSELDI-TOF-MS may be a useful tool in high-risk population screening for endometriosis. The identification and application of the biomarkers need to further study.