Inhibiting severe acute respiratory syndrome-associated coronavirus by small interfering RNA.
- Author:
Renli ZHANG
1
;
Zhongmin GUO
;
Jiahai LU
;
Jinxiu MENG
;
Canquan ZHOU
;
Ximei ZHAN
;
Bing HUANG
;
Xinbing YU
;
Min HUANG
;
Xinghua PAN
;
Wenhua LING
;
Xigu CHEN
;
Zhuoyue WAN
;
Huanying ZHENG
;
Xinge YAN
;
Yifei WANG
;
Yanchao RAN
;
Xinjian LIU
;
Junxin MA
;
Chengyu WANG
;
Biliang ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cercopithecus aethiops; RNA, Small Interfering; pharmacology; SARS Virus; drug effects; Transfection; Vero Cells; Virus Replication; drug effects
- From: Chinese Medical Journal 2003;116(8):1262-1264
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo evaluate the effectiveness of small interfering RNA (siRNA) on inhibiting severe acute respiratory syndrome (SARS)-associated coronavirus replication, and to lay bases for the future clinical application of siRNA for the treatment of viral infectious diseases.
METHODSVero-E6 cells was transfected with siRNA before SARS virus infection, and the effectiveness of siRNA interference was evaluated by observing the cytopathic effect (CPE) on Vero-E6 cells.
RESULTSFive pairs of siRNA showed ability to reduce CPE dose dependently, and two of them had the best effect.
CONCLUSIONsiRNA may be effective in inhibiting SARS-associated coronavirus replication.
